Software:Article Title: Danggui Shaoyao San ameliorates neuroinflammation in a D-galactose-induced Alzheimer’s disease rat model by suppressing the JAK2/STAT3 pathway and modulating Th17/Treg -related immune dysregulation
Article Snippet: .. Other reagents included sodium pentobarbital (Merck KGaA, Germany, #P3761), donepezil hydrochloride (MCE, United States, #HY-B0034), D-galactose (Sigma-Aldrich, Shanghai, China, #V900922), primary antibodies targeting STAT3 and Foxp3 (Proteintech, Wuhan, China, #102532-AP, #22228-1-AP), ROR-γt and p-STAT3-Y705 antibodies (HUABIO, Hangzhou, China, #HA722121, #ET1603-40), JAK2 antibody (Aifang, Hunan, China, #AFRM99330), p-JAK2-Y1007/1008 antibody (ABclonal, Wuhan, China, #AP0531), β-actin antibody (Affinity Biosciences, China, #AF7018), postsynaptic density protein 95 (PSD95) and synaptophysin I antibody (abcam, Cambridge, United Kingdom, #ab18258,#ab254349), secondary antibodies (Elabscience, Wuhan, China, #E-AB-1003), Iba1 antibody (Wako Pure Chemical Industries, Ltd., Japan, #011-27991), primers for β-actin, IL-1β, IL-6, etc., were designed using Primer 6.0 software based on sequences from the NCBI database and synthesized by Sangon Biotech (Shanghai), China, reverse transcription kit (Novoprotein, Suzhou, China, #E047-01B), SYBR Mixture premix and RIPA protein lysis buffer (Comwin Biotech, Jiangsu, China, #CW3008M, #CW2333S), ELISA kits (Jianglai Biotechnology, Shanghai, China, #JL13427, #JL20880), BCA protein concentration assay kit (Elabscience Biotechnology, Wuhan, China, #E-BC-K318-M), TRIzol, SlowFadeTM Gold Antifade Mountant with DAPI, and donkey anti-goat Alexa Fluor 647 fluorescent secondary antibody (red) (Thermo Fisher Scientific, United States, #15596-026, # S36942 , #A32849), universal two-step detection kit (Zhongshan Golden Bridge, Beijing, China, #PV-9000), Toluidine blue, Triton X-100, and antifade mounting medium with DAPI (Solarbio Science & Technology Co., Ltd., Beijing, China, #G3663, #T8200, #S2110), hematoxylin staining solution and eosin staining solution (Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China, #ZLI-9610, #ZLI-9613), Fixation/permeabilization kits, dyes, and antibodies against CD4, Foxp3, and IL-17A for phenotypic analysis of Th17 and Treg cells were purchased from BD Pharmingen (United States), detailed information is provided in . ..
Synthesized:Article Title: Danggui Shaoyao San ameliorates neuroinflammation in a D-galactose-induced Alzheimer’s disease rat model by suppressing the JAK2/STAT3 pathway and modulating Th17/Treg -related immune dysregulation
Article Snippet: .. Other reagents included sodium pentobarbital (Merck KGaA, Germany, #P3761), donepezil hydrochloride (MCE, United States, #HY-B0034), D-galactose (Sigma-Aldrich, Shanghai, China, #V900922), primary antibodies targeting STAT3 and Foxp3 (Proteintech, Wuhan, China, #102532-AP, #22228-1-AP), ROR-γt and p-STAT3-Y705 antibodies (HUABIO, Hangzhou, China, #HA722121, #ET1603-40), JAK2 antibody (Aifang, Hunan, China, #AFRM99330), p-JAK2-Y1007/1008 antibody (ABclonal, Wuhan, China, #AP0531), β-actin antibody (Affinity Biosciences, China, #AF7018), postsynaptic density protein 95 (PSD95) and synaptophysin I antibody (abcam, Cambridge, United Kingdom, #ab18258,#ab254349), secondary antibodies (Elabscience, Wuhan, China, #E-AB-1003), Iba1 antibody (Wako Pure Chemical Industries, Ltd., Japan, #011-27991), primers for β-actin, IL-1β, IL-6, etc., were designed using Primer 6.0 software based on sequences from the NCBI database and synthesized by Sangon Biotech (Shanghai), China, reverse transcription kit (Novoprotein, Suzhou, China, #E047-01B), SYBR Mixture premix and RIPA protein lysis buffer (Comwin Biotech, Jiangsu, China, #CW3008M, #CW2333S), ELISA kits (Jianglai Biotechnology, Shanghai, China, #JL13427, #JL20880), BCA protein concentration assay kit (Elabscience Biotechnology, Wuhan, China, #E-BC-K318-M), TRIzol, SlowFadeTM Gold Antifade Mountant with DAPI, and donkey anti-goat Alexa Fluor 647 fluorescent secondary antibody (red) (Thermo Fisher Scientific, United States, #15596-026, # S36942 , #A32849), universal two-step detection kit (Zhongshan Golden Bridge, Beijing, China, #PV-9000), Toluidine blue, Triton X-100, and antifade mounting medium with DAPI (Solarbio Science & Technology Co., Ltd., Beijing, China, #G3663, #T8200, #S2110), hematoxylin staining solution and eosin staining solution (Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China, #ZLI-9610, #ZLI-9613), Fixation/permeabilization kits, dyes, and antibodies against CD4, Foxp3, and IL-17A for phenotypic analysis of Th17 and Treg cells were purchased from BD Pharmingen (United States), detailed information is provided in . ..
Reverse Transcription:Article Title: Danggui Shaoyao San ameliorates neuroinflammation in a D-galactose-induced Alzheimer’s disease rat model by suppressing the JAK2/STAT3 pathway and modulating Th17/Treg -related immune dysregulation
Article Snippet: .. Other reagents included sodium pentobarbital (Merck KGaA, Germany, #P3761), donepezil hydrochloride (MCE, United States, #HY-B0034), D-galactose (Sigma-Aldrich, Shanghai, China, #V900922), primary antibodies targeting STAT3 and Foxp3 (Proteintech, Wuhan, China, #102532-AP, #22228-1-AP), ROR-γt and p-STAT3-Y705 antibodies (HUABIO, Hangzhou, China, #HA722121, #ET1603-40), JAK2 antibody (Aifang, Hunan, China, #AFRM99330), p-JAK2-Y1007/1008 antibody (ABclonal, Wuhan, China, #AP0531), β-actin antibody (Affinity Biosciences, China, #AF7018), postsynaptic density protein 95 (PSD95) and synaptophysin I antibody (abcam, Cambridge, United Kingdom, #ab18258,#ab254349), secondary antibodies (Elabscience, Wuhan, China, #E-AB-1003), Iba1 antibody (Wako Pure Chemical Industries, Ltd., Japan, #011-27991), primers for β-actin, IL-1β, IL-6, etc., were designed using Primer 6.0 software based on sequences from the NCBI database and synthesized by Sangon Biotech (Shanghai), China, reverse transcription kit (Novoprotein, Suzhou, China, #E047-01B), SYBR Mixture premix and RIPA protein lysis buffer (Comwin Biotech, Jiangsu, China, #CW3008M, #CW2333S), ELISA kits (Jianglai Biotechnology, Shanghai, China, #JL13427, #JL20880), BCA protein concentration assay kit (Elabscience Biotechnology, Wuhan, China, #E-BC-K318-M), TRIzol, SlowFadeTM Gold Antifade Mountant with DAPI, and donkey anti-goat Alexa Fluor 647 fluorescent secondary antibody (red) (Thermo Fisher Scientific, United States, #15596-026, # S36942 , #A32849), universal two-step detection kit (Zhongshan Golden Bridge, Beijing, China, #PV-9000), Toluidine blue, Triton X-100, and antifade mounting medium with DAPI (Solarbio Science & Technology Co., Ltd., Beijing, China, #G3663, #T8200, #S2110), hematoxylin staining solution and eosin staining solution (Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China, #ZLI-9610, #ZLI-9613), Fixation/permeabilization kits, dyes, and antibodies against CD4, Foxp3, and IL-17A for phenotypic analysis of Th17 and Treg cells were purchased from BD Pharmingen (United States), detailed information is provided in . ..
Lysis:Article Title: Danggui Shaoyao San ameliorates neuroinflammation in a D-galactose-induced Alzheimer’s disease rat model by suppressing the JAK2/STAT3 pathway and modulating Th17/Treg -related immune dysregulation
Article Snippet: .. Other reagents included sodium pentobarbital (Merck KGaA, Germany, #P3761), donepezil hydrochloride (MCE, United States, #HY-B0034), D-galactose (Sigma-Aldrich, Shanghai, China, #V900922), primary antibodies targeting STAT3 and Foxp3 (Proteintech, Wuhan, China, #102532-AP, #22228-1-AP), ROR-γt and p-STAT3-Y705 antibodies (HUABIO, Hangzhou, China, #HA722121, #ET1603-40), JAK2 antibody (Aifang, Hunan, China, #AFRM99330), p-JAK2-Y1007/1008 antibody (ABclonal, Wuhan, China, #AP0531), β-actin antibody (Affinity Biosciences, China, #AF7018), postsynaptic density protein 95 (PSD95) and synaptophysin I antibody (abcam, Cambridge, United Kingdom, #ab18258,#ab254349), secondary antibodies (Elabscience, Wuhan, China, #E-AB-1003), Iba1 antibody (Wako Pure Chemical Industries, Ltd., Japan, #011-27991), primers for β-actin, IL-1β, IL-6, etc., were designed using Primer 6.0 software based on sequences from the NCBI database and synthesized by Sangon Biotech (Shanghai), China, reverse transcription kit (Novoprotein, Suzhou, China, #E047-01B), SYBR Mixture premix and RIPA protein lysis buffer (Comwin Biotech, Jiangsu, China, #CW3008M, #CW2333S), ELISA kits (Jianglai Biotechnology, Shanghai, China, #JL13427, #JL20880), BCA protein concentration assay kit (Elabscience Biotechnology, Wuhan, China, #E-BC-K318-M), TRIzol, SlowFadeTM Gold Antifade Mountant with DAPI, and donkey anti-goat Alexa Fluor 647 fluorescent secondary antibody (red) (Thermo Fisher Scientific, United States, #15596-026, # S36942 , #A32849), universal two-step detection kit (Zhongshan Golden Bridge, Beijing, China, #PV-9000), Toluidine blue, Triton X-100, and antifade mounting medium with DAPI (Solarbio Science & Technology Co., Ltd., Beijing, China, #G3663, #T8200, #S2110), hematoxylin staining solution and eosin staining solution (Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China, #ZLI-9610, #ZLI-9613), Fixation/permeabilization kits, dyes, and antibodies against CD4, Foxp3, and IL-17A for phenotypic analysis of Th17 and Treg cells were purchased from BD Pharmingen (United States), detailed information is provided in . ..
Enzyme-linked Immunosorbent Assay:Article Title: Danggui Shaoyao San ameliorates neuroinflammation in a D-galactose-induced Alzheimer’s disease rat model by suppressing the JAK2/STAT3 pathway and modulating Th17/Treg -related immune dysregulation
Article Snippet: .. Other reagents included sodium pentobarbital (Merck KGaA, Germany, #P3761), donepezil hydrochloride (MCE, United States, #HY-B0034), D-galactose (Sigma-Aldrich, Shanghai, China, #V900922), primary antibodies targeting STAT3 and Foxp3 (Proteintech, Wuhan, China, #102532-AP, #22228-1-AP), ROR-γt and p-STAT3-Y705 antibodies (HUABIO, Hangzhou, China, #HA722121, #ET1603-40), JAK2 antibody (Aifang, Hunan, China, #AFRM99330), p-JAK2-Y1007/1008 antibody (ABclonal, Wuhan, China, #AP0531), β-actin antibody (Affinity Biosciences, China, #AF7018), postsynaptic density protein 95 (PSD95) and synaptophysin I antibody (abcam, Cambridge, United Kingdom, #ab18258,#ab254349), secondary antibodies (Elabscience, Wuhan, China, #E-AB-1003), Iba1 antibody (Wako Pure Chemical Industries, Ltd., Japan, #011-27991), primers for β-actin, IL-1β, IL-6, etc., were designed using Primer 6.0 software based on sequences from the NCBI database and synthesized by Sangon Biotech (Shanghai), China, reverse transcription kit (Novoprotein, Suzhou, China, #E047-01B), SYBR Mixture premix and RIPA protein lysis buffer (Comwin Biotech, Jiangsu, China, #CW3008M, #CW2333S), ELISA kits (Jianglai Biotechnology, Shanghai, China, #JL13427, #JL20880), BCA protein concentration assay kit (Elabscience Biotechnology, Wuhan, China, #E-BC-K318-M), TRIzol, SlowFadeTM Gold Antifade Mountant with DAPI, and donkey anti-goat Alexa Fluor 647 fluorescent secondary antibody (red) (Thermo Fisher Scientific, United States, #15596-026, # S36942 , #A32849), universal two-step detection kit (Zhongshan Golden Bridge, Beijing, China, #PV-9000), Toluidine blue, Triton X-100, and antifade mounting medium with DAPI (Solarbio Science & Technology Co., Ltd., Beijing, China, #G3663, #T8200, #S2110), hematoxylin staining solution and eosin staining solution (Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China, #ZLI-9610, #ZLI-9613), Fixation/permeabilization kits, dyes, and antibodies against CD4, Foxp3, and IL-17A for phenotypic analysis of Th17 and Treg cells were purchased from BD Pharmingen (United States), detailed information is provided in . ..
Protein Concentration:Article Title: Danggui Shaoyao San ameliorates neuroinflammation in a D-galactose-induced Alzheimer’s disease rat model by suppressing the JAK2/STAT3 pathway and modulating Th17/Treg -related immune dysregulation
Article Snippet: .. Other reagents included sodium pentobarbital (Merck KGaA, Germany, #P3761), donepezil hydrochloride (MCE, United States, #HY-B0034), D-galactose (Sigma-Aldrich, Shanghai, China, #V900922), primary antibodies targeting STAT3 and Foxp3 (Proteintech, Wuhan, China, #102532-AP, #22228-1-AP), ROR-γt and p-STAT3-Y705 antibodies (HUABIO, Hangzhou, China, #HA722121, #ET1603-40), JAK2 antibody (Aifang, Hunan, China, #AFRM99330), p-JAK2-Y1007/1008 antibody (ABclonal, Wuhan, China, #AP0531), β-actin antibody (Affinity Biosciences, China, #AF7018), postsynaptic density protein 95 (PSD95) and synaptophysin I antibody (abcam, Cambridge, United Kingdom, #ab18258,#ab254349), secondary antibodies (Elabscience, Wuhan, China, #E-AB-1003), Iba1 antibody (Wako Pure Chemical Industries, Ltd., Japan, #011-27991), primers for β-actin, IL-1β, IL-6, etc., were designed using Primer 6.0 software based on sequences from the NCBI database and synthesized by Sangon Biotech (Shanghai), China, reverse transcription kit (Novoprotein, Suzhou, China, #E047-01B), SYBR Mixture premix and RIPA protein lysis buffer (Comwin Biotech, Jiangsu, China, #CW3008M, #CW2333S), ELISA kits (Jianglai Biotechnology, Shanghai, China, #JL13427, #JL20880), BCA protein concentration assay kit (Elabscience Biotechnology, Wuhan, China, #E-BC-K318-M), TRIzol, SlowFadeTM Gold Antifade Mountant with DAPI, and donkey anti-goat Alexa Fluor 647 fluorescent secondary antibody (red) (Thermo Fisher Scientific, United States, #15596-026, # S36942 , #A32849), universal two-step detection kit (Zhongshan Golden Bridge, Beijing, China, #PV-9000), Toluidine blue, Triton X-100, and antifade mounting medium with DAPI (Solarbio Science & Technology Co., Ltd., Beijing, China, #G3663, #T8200, #S2110), hematoxylin staining solution and eosin staining solution (Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China, #ZLI-9610, #ZLI-9613), Fixation/permeabilization kits, dyes, and antibodies against CD4, Foxp3, and IL-17A for phenotypic analysis of Th17 and Treg cells were purchased from BD Pharmingen (United States), detailed information is provided in . ..
Staining:Article Title: Danggui Shaoyao San ameliorates neuroinflammation in a D-galactose-induced Alzheimer’s disease rat model by suppressing the JAK2/STAT3 pathway and modulating Th17/Treg -related immune dysregulation
Article Snippet: .. Other reagents included sodium pentobarbital (Merck KGaA, Germany, #P3761), donepezil hydrochloride (MCE, United States, #HY-B0034), D-galactose (Sigma-Aldrich, Shanghai, China, #V900922), primary antibodies targeting STAT3 and Foxp3 (Proteintech, Wuhan, China, #102532-AP, #22228-1-AP), ROR-γt and p-STAT3-Y705 antibodies (HUABIO, Hangzhou, China, #HA722121, #ET1603-40), JAK2 antibody (Aifang, Hunan, China, #AFRM99330), p-JAK2-Y1007/1008 antibody (ABclonal, Wuhan, China, #AP0531), β-actin antibody (Affinity Biosciences, China, #AF7018), postsynaptic density protein 95 (PSD95) and synaptophysin I antibody (abcam, Cambridge, United Kingdom, #ab18258,#ab254349), secondary antibodies (Elabscience, Wuhan, China, #E-AB-1003), Iba1 antibody (Wako Pure Chemical Industries, Ltd., Japan, #011-27991), primers for β-actin, IL-1β, IL-6, etc., were designed using Primer 6.0 software based on sequences from the NCBI database and synthesized by Sangon Biotech (Shanghai), China, reverse transcription kit (Novoprotein, Suzhou, China, #E047-01B), SYBR Mixture premix and RIPA protein lysis buffer (Comwin Biotech, Jiangsu, China, #CW3008M, #CW2333S), ELISA kits (Jianglai Biotechnology, Shanghai, China, #JL13427, #JL20880), BCA protein concentration assay kit (Elabscience Biotechnology, Wuhan, China, #E-BC-K318-M), TRIzol, SlowFadeTM Gold Antifade Mountant with DAPI, and donkey anti-goat Alexa Fluor 647 fluorescent secondary antibody (red) (Thermo Fisher Scientific, United States, #15596-026, # S36942 , #A32849), universal two-step detection kit (Zhongshan Golden Bridge, Beijing, China, #PV-9000), Toluidine blue, Triton X-100, and antifade mounting medium with DAPI (Solarbio Science & Technology Co., Ltd., Beijing, China, #G3663, #T8200, #S2110), hematoxylin staining solution and eosin staining solution (Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China, #ZLI-9610, #ZLI-9613), Fixation/permeabilization kits, dyes, and antibodies against CD4, Foxp3, and IL-17A for phenotypic analysis of Th17 and Treg cells were purchased from BD Pharmingen (United States), detailed information is provided in . ..
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